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Products >  Molecular_Biology >  Modifying_Enzymes >  Nucleases >  BAL_31_Nuclease

BAL 31 Nuclease

BAL 31 Nuclease has two types of activity. It acts as an exonuclease, degrading double-stranded DNA and RNA from both 5'- and 3'-ends. It also possesses single-strand endonuclease activity. BAL 31 Nuclease is supplied in 20 mM Tris-HCl (pH 8.0), 100 mM NaCl, 5 mM CaCl2, 5 mM MgCl2, 1 mM EDTA and 50% glycerol.
At-A-Glance Documents

Applications

  • Generating deletions from both ends of DNA fragments

Source

Alteromonas espejiana BAL 31

Storage

–20°C

Buffer

Supplied with 2X Reaction Buffer [40 mM Tris-HCl (pH 8.0), 1200 mM NaCl, 24 mM MgCl2, 24 mM CaCl2 and 2 mM EDTA].

References

  1. Gray, Jr., H.B. et al. (1981) Gene Amplif. Anal. 2:169-203.

Unit Definition

One unit is defined as the amount of enzyme that produces 1 µg of acid-soluble products from heat-denatured calf thymus DNA in 1 minute at 30°C and pH 8.0.

Concentration

1–3 U/µL


 
 
Products
Cat. # Product Package Size Price # of Units Select
2510A BAL 31 Nuclease 50 Units $78.00
2510B BAL 31 Nuclease 250 Units $309.00
 

 


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