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Products >  Molecular_Biology >  Modifying_Enzymes >  Ligases >  T4_RNA_Ligase

T4 RNA Ligase

T4 RNA Ligase catalyzes the formation of phosphodiester bonds between single-stranded RNA or DNA containing 5'-phosphate and 3'-hydroxyl termini. The enzyme requires ATP for activity. The T4 RNA Ligase activity is highest for RNA-RNA ligation, lower for RNA-DNA ligation and lowest for DNA-DNA ligation. 3' end labeling is often completed using 5'-[32P]pCp.

The enzyme is supplied in a buffer of 20 mM Tris-HCl (pH 7.5), 50 mM NaCl, 1 mm DTT, 0.1 mM EDTA and 50% glycerol.

 
At-A-Glance Documents Images & Data

Applications

  • Ligation of single-stranded RNA or DNA
  • 3'-end labeling of single stranded RNA or DNA

Components

2050A: 1000 Units

2050B: 5 x 2050A (5000 Units)

Source

E. coli B strain infected with T4 am N82

Storage

–20°C

Concentration

10–50 U/µL

References

  1. Romaniuk, P.J. and Uhlenbeck, O.C. (1983) Methods Enzymol. 100:52-59.
  2. England, T.E. et al. (1980) Methods Enzymol. 65:65-74.

Buffer

Supplied with 10X Reaction Buffer [500 mM Tris-HCl (pH 7.5), 100 mM MgCl2, 100 mM DTT and 10 mM ATP].

Unit Definition

One unit is defined as the amount of enzyme that converts 1 pmol of [5'-32P]pCp into its acid-insoluble form in 10 minutes at 5°C, using oligo(A) as the substrate during 3' end labeling of RNA.


 
 
Products
Cat. # Product Package Size Price # of Units Select
2050A T4 RNA Ligase 1,000 Units $157.00
2050B T4 RNA Ligase 5,000 Units $629.00
 

 


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