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Products >  Molecular_Biology >  Modifying_Enzymes >  Ligases >  T4_DNA_Ligase

T4 DNA Ligase

T4 DNA Ligase catalyzes the formation of phosphodiester bonds between double-stranded DNA fragments with 3'-OH and 5'-phosphate ends, in the presence of ATP. T4 DNA Ligase is supplied in 10 mM Tris-HCl (pH 7.5), 50 mM KCl, 10 mM 2-mercaptoethanol, 0.1 mM EDTA and 50% glycerol.
At-A-Glance Documents

Applications

  • Joining two fragments of DNA (blunt-ended or with compatible overhangs)
  • Adding linkers to blunt-ended DNA

Components

2011A is comprised of 200 Weiss units
2011B is comprised of 1000 Weiss units

Source

Recombinant E. coli strain

Storage

–20°C

Unit Definition

One ligation unit is defined as the amount of enzyme that ligates >90% of 6 µg of lambda DNA-Hind III fragments in a 20 µL reaction volume in 30 minutes at 16°C. One unit of the enzyme corresponds to 0.008 Weiss units by the ATP-PPi exchange reaction in 66 mM Tris-HCl (pH 7.6), 6.6 mM MgCl2, 10 mM DTT, 66 µM ATP and 3.3 µM [32P]Na4P2O7.

Buffer

Supplied with 10X Reaction Buffer [660 mM Tris-HCl (pH 7.6), 66 mM MgCl2, 100 mM DTT and 1 mM ATP].

Concentration

350 U/µL (2.8 Weiss units/µL)


 
 
Products
Cat. # Product Package Size Price # of Units Select
2011A T4 DNA Ligase 25,000 Units $44.00
2011B T4 DNA Ligase 125,000 Units $176.00
 

 


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