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Products >  Molecular_Biology >  Modifying_Enzymes >  Ligases >  DNA_Ligase_E_coli

E. coli DNA Ligase

E. coli DNA Ligase catalyzes the formation of phosphodiester bonds between double-stranded DNA fragments containing juxtaposed 5'-phosphate termini and 3'-hydroxyl termini in the presence of the NAD cofactor. Both T4 DNA Ligase and E.coli DNA Ligase are used in various gene cloning experiments;  however, unlike T4 DNA Ligase, E. coli DNA Ligase can only catalyze ligation of cohesive end-containing DNA fragments via standard reaction conditions. E. coli DNA Ligase is supplied in a buffer containing 10 mM potassium phosphate (pH 7.5), 50 mM KCl, 1 mM DTT, 1 mM EDTA and 50% glycerol.

At-A-Glance Documents Images & Data

Applications

  • Gene cloning
  • Ligation
  • Phosphodiester bond formation

Source

Recombinant E. coli

Storage

–20°C

Unit Definition

One unit is defined as the amount of enzyme that ligates >90% of 6 µg of Hind III-restricted DNA fragments in 30 minutes and 16°C (using a 20 ul reaction volume).

References

  1. Panasenko, S.M. et al. (1978) J. Biol. Chem. 253:4590-4592.

Concentration

50-100 U/µL




 
 
Products
Cat. # Product Package Size Price # of Units Select
2160A E. coli DNA Ligase 1,000 Units $80.00
2160B E. coli DNA Ligase 5,000 Units $315.00
 

 



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