Takara
login | register | order now | view cart  
Products
Molecular Biology
Cloning, Mapping and cDNA Synthesis
DNA Labeling Kits
Gene Transfer and Expression
Instrumentation
Modifying Enzymes
    DNA Polymerase
    Kinases and RTs
    Ligases
         DNA Ligase (E. coli)
         T4 DNA Ligase
         T4 RNA Ligase
    Nucleases
    Phosphatases
    RNA Polymerase
    RNase Inhibitor
Molecular Biology Reagents
Nucleic Acids
Plant Products
Restriction Enzymes
RNA, Protein and DNA Extraction
Sequencing, Mutagenesis and Mutation


Products >  Molecular_Biology >  Modifying_Enzymes >  Ligases >  DNA_Ligase_E_coli

DNA Ligase (E. coli)

E. coli DNA Ligase catalyzes the formation of phosphodiester bonds between double-stranded DNA fragments with 3'-OH and 5'-phosphate ends, in the presence of NAD as the cofactor. Unlike T4 DNA Ligase, E. coli DNA Ligase can only ligate DNA fragments with cohesive ends under standard reaction conditions. E. coli DNA Ligase is supplied in 10 mM potassium phosphate (pH 7.5), 50 mM KCl, 1 mM DTT, 1 mM EDTA and 50% glycerol.
At-A-Glance Documents

Applications

  • Joining two fragments of DNA with cohesive ends

Source

Recombinant E. coli

Storage

–20°C

Unit Definition

One unit is defined as the amount of enzyme that ligates >90% of 6 µg of DNA-Hind III fragments in a 20 µL reaction volume in 30 minutes at 16°C.

References

  1. Panasenko, S.M. et al. (1978) J. Biol. Chem. 253:4590-2.

Concentration

50-100 U/µL


 
 
Products
Cat. # Product Package Size Price # of Units Select
2160A E. coli DNA Ligase 1,000 Units $78.00
2160B E. coli DNA Ligase 5,000 Units $309.00
 

 


Clontech is a Takara Bio Company © 2012 Privacy Policy | Terms & Conditions | TRUSTe