Applications - Tailing Reactions to add Complementary Homopolymer Tails to Vectors and cDNA, such as in the Okayama-Berg Method
- Labeling the 3'-Ends of DNA Fragments using [32P]dNTP or [32P]ddNTP
Supplied Buffer - 5X TdT Buffer [500 mM HEPES (pH 7.2), 40 mM MgCl2, 0.5 mM DTT]
- 0.1% BSA
Source E. coli recombinant
Purity Endonuclease, Exonuclease and Nickase activity were not detected as judged via gel electrophoresis.
Storage –20°C
Unit Definition One unit is the amount of enzyme that incorporates 1 nmol of [3H]dATP into acid-insoluble products in 1 hour at 37°C and pH 7.2, with calf thymus, DNA that is treated with DNase and heat-denatured (activated calf thymus DNA) as the initiator.
Concentration 7–15 units/µL
Form 60 mM potassium phosphate (pH 7.2), 150 mM KCl, 1 mM 2-mercaptoethanol, 50% glycerol
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