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Products >  Molecular_Biology >  Modifying_Enzymes >  Kinases_and_RT >  Terminal_Deoxynucleotidyl_Transferase

Terminal Deoxynucleotidyl Transferase (TdT)

Terminal Deoxynucleotidyl Transferase (TdT) catalyzes the incorporation of single deoxynucleotides into the 3'-OH terminus of single- or double-stranded DNA, making it an effective agent in 3' end labeling applications such as terminal dUTP nick end labeling (TUNEL). Terminal Deoxynucleotidyl Transferase (TdT) requires an oligodeoxynucleotide of at least three bases as a primer but is not template-dependent.  

 

At-A-Glance Documents Images & Data

Applications

  • Okayama-Berg methods involving complementary homopolymer tailing of vectors and cDNA
  • 3'-end labeling of DNA Fragments using [32P]dNTP or [32P]ddNTP
  • TUNEL

Supplied Buffer

  • 5X TdT Buffer [500 mM HEPES (pH 7.2), 40 mM MgCl2, 0.5 mM DTT]
  • 0.1% BSA

Source

Recombinant E. coli

Purity

Endonuclease, exonuclease and nickase activities were not detected via gel electrophoresis.

Storage

–20°C

Unit Definition

One unit is the amount of enzyme that incorporates 1 nmol of [3H]dATP into acid-insoluble product in 1 hour at 37°C and pH 7.2 using activated, heat-denatured and DNase-treated calf thymus DNA as the initiator.

Concentration

7–15 units/µL

Form

60 mM potassium phosphate (pH 7.2), 150 mM KCl, 1 mM 2-mercaptoethanol, 50% glycerol




 
 
Products
Cat. # Product Package Size Price # of Units Select
2230A Terminal Deoxynucleotidyl Transferase 300 Units $43.00
2230B Terminal Deoxynucleotidyl Transferase 1,500 Units $171.00
 

 



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