- Okayama-Berg methods involving complementary homopolymer tailing of vectors and cDNA
- 3'-end labeling of DNA Fragments using [32P]dNTP or [32P]ddNTP
- 5X TdT Buffer [500 mM HEPES (pH 7.2), 40 mM MgCl2, 0.5 mM DTT]
- 0.1% BSA
Recombinant E. coli
Endonuclease, exonuclease and nickase activities were not detected via gel electrophoresis.
One unit is the amount of enzyme that incorporates 1 nmol of [3H]dATP into acid-insoluble product in 1 hour at 37°C and pH 7.2 using activated, heat-denatured and DNase-treated calf thymus DNA as the initiator.
60 mM potassium phosphate (pH 7.2), 150 mM KCl, 1 mM 2-mercaptoethanol, 50% glycerol