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Products >  Molecular_Biology >  Modifying_Enzymes >  Kinases_and_RT >  AMV_Reverse_Transcriptase_XL

AMV Reverse Transcriptase XL

AMV Reverse Transcriptase XL synthesizes complementary full-length cDNA from single-stranded RNA, DNA or RNA-DNA hybrid template. The enzyme requires a primer to initiate synthesis and Mg2+ or Mn2+ for activity. AMV Reverse Transcriptase XL is positive for 5'->3' ribonuclease H activity and can be used for a number of applications including reverse transcription.

AMV Reverse Transcriptase XL is highly purified, free of extraneous nucleases and is qualified for use in full-length cDNA synthesis and reverse transcription using RNA templates that are up to 10 kb in length. It can be used over a temperature range of 37–55°C but maximal yield of full-length cDNA product is achieved between 41–44°C.

At-A-Glance Documents Images & Data

Applications

  • Reverse transcription
  • Full-length cDNA synthesis
  • Primer extension
  • Preparation of DNA probes

Source

Avian Myeloblastosis Virus (Manufactured by Life Science Co.)

Storage

–80°C. Avoid repeated freeze-thaw. Can be stored at –20° for up to 6 months.

Concentration

5 U/µL

Unit Definition

One unit is defined as the amount of AMV RT XL, required to catalyze the incorporation of one nmol of [3H] dTMP into acid-insoluble products in 10 minutes at 37°C using poly (rA) • oligo(dT)12-18 as the template-primer.

 




 
 
Products
Cat. # Product Package Size Price # of Units Select
2630A AMV Reverse Transcriptase XL for RT-PCR 250 Units $190.00
 

 


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