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Products >  Molecular_Biology >  Modifying_Enzymes >  DNA_Polymerase >  T4_DNA_Polymerase

T4 DNA Polymerase

T4 DNA Polymerase catalyzes the 5'–>3' incorporation of nucleotides into double-stranded DNA using single-stranded and primed DNA as a template. It possesses strong 3'–>5' exonuclease (proofreading) activity but does not exhibit 5'–>3' exonuclease activity. Typical T4 DNA polymerase protocols include 5' DNA overhang blunting as well as the generation of blunt double-stranded DNA from double-stranded DNA containing 3' overhangs. These DNA products are often used in blunt cloning. T4 DNA Polymerase is supplied in a buffer of 200 mM potassium phosphate (pH 6.5), 10 mM DTT and 50% glycerol.


 
At-A-Glance Documents Images & Data Resources

Applications

  • Generation of blunt double-stranded DNA from DNA containing 5' overhangs
  • Generation of blunt double-stranded DNA from DNA containing 3' overhangs
  • 5'-end or 3'-end labeling of double-stranded DNA
  • In vitro mutagenesis

Components

2040A: 100 Units

2040B: 5 x 2040A (500 Units)

Source

Recombinant E. coli

Storage

–20°C

Buffer

Supplied with 10X Reaction Buffer [330 mM Tris-acetate (pH 7.9), 660 mM potassium acetate, 100 mM magnesium acetate and 5 mM DTT] and 0.1% BSA.

References

  1. Kunkel, T.A.et al. (1987) Methods Enzymol. 154:367-382.
  2. Deen, K. C.et al. (1983) Anal. Biochem. 135:456-465.
  3. Wartell, R.M. and Reznikoff, W. S. (1980) Gene 9:307-319.

Unit Definition

One unit is defined as the amount of enzyme that catalyzes the incorporation of 10 nmol of total nucleotides into acid-insoluble product in 30 minutes at 37°C and pH 8.8 using heat-denatured calf thymus DNA as the template-primer.

Concentration

2–5 U/µL


 
 
Products
Cat. # Product Package Size Price # of Units Select
2040A T4 DNA Polymerase 100 Units $81.00
2040B T4 DNA Polymerase 500 Units $318.00
 

 


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