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Products >  Molecular_Biology >  Modifying_Enzymes >  DNA_Polymerase >  T4_DNA_Polymerase

T4 DNA Polymerase

T4 DNA Polymerase catalyzes the incorporation of nucleotides into double-stranded DNA in a 5'–>3' direction. It possesses a strong 3'–>5' exonuclease (proofreading) activity, but does not exhibit 5'–>3' exonuclease activity. T4 DNA Polymerase is supplied in 200 mM potassium phosphate (pH 6.5), 10 mM DTT and 50% glycerol.
At-A-Glance Documents

Applications

  • Creating blunt ends from protruding ends in double-stranded DNA fragments
  • 3'-end labeling of double-stranded DNA
  • Site-directed mutagenesis

Components

The B size consists of 5 A sizes (100U)

Source

Recombinant E. coli

Storage

–20°C

Buffer

Supplied with 10X Reaction Buffer [330 mM Tris-acetate (pH 7.9), 660 mM potassium acetate, 100 mM magnesium acetate and 5 mM DTT] and 0.1% BSA.

References

  1. Kunkel, T.A.et al. (1987) Methods Enzymol. 154:367-82.
  2. Deen, K. C.et al. (1983) Anal. Biochem. 135:456-65.
  3. Wartell, R.M. and Reznikoff, W. S. (1980) Gene 9:307-19.

Unit Definition

One unit is defined as the amount of enzyme that catalyzes the incorporation of 10 nmol of total nucleotides into acid-insoluble products in 30 minutes at 37°C and pH 8.8, using heat-denatured calf thymus DNA as the template-primer.

Concentration

2–5 U/µL


 
 
Products
Cat. # Product Package Size Price # of Units Select
2040A T4 DNA Polymerase 100 Units $79.00
2040B T4 DNA Polymerase 500 Units $312.00
 

 


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