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Products >  Molecular_Biology >  Modifying_Enzymes >  DNA_Polymerase >  Poly_A_Polymerase

Poly(A) Polymerase

Poly(A) Polymerase catalyzes the incorporation of adenine residues into the 3'-termini of RNA, effectively adding a poly(A) tail to RNA. The enzyme uses single-stranded RNA as a primer during poly(A) tailing. Poly(A) Polymerase is supplied in a buffer of 25 mM Tris-HCl (pH 7.9), 500 mM NaCl, 1 mM EDTA, 0.1 mM DTT and 50% glycerol.

At-A-Glance Documents Images & Data

Applications

  • Poly(A) tailing of RNA
  • 3'-end RNA labeling
  • Adding a poly(A) tail to RNA  

Components

2180A: (20 Units)

2180B: 5 x 2180A (100 Units)

Source

E. coli B

Storage

–20°C

Concentration

0.2–2 U/µL

References

  1. Krug, M.S. and Berger, S.L. (1987) Methods Enzymol. 152:316-325.
  2. Winter, G. and Brownlee, G.G. (1978) Nucleic Acids Res. 5:3129-3139.
  3. Gething, M.J. et al. (1980) Nature 287:301-306. 

Unit Definition

One unit is defined as the amount of enzyme that incorporates 1 nmol of AMP into tRNA in 10 minutes at 37°C and pH 7.9 using ATP as the substrate.




 
 
Products
Cat. # Product Package Size Price # of Units Select
2180A Poly(A) Polymerase 20 Units $80.00
2180B Poly(A) Polymerase 100 Units $315.00
 

 


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