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Products >  Molecular_Biology >  Modifying_Enzymes >  DNA_Polymerase >  Poly_A_Polymerase

Poly(A) Polymerase

Poly(A) Polymerase catalyzes the incorporation of adenine residues into the 3'-termini of RNA. The enzyme uses single-stranded RNA as a primer. Poly(A) Polymerase is supplied in 25 mM Tris-HCl (pH 7.9), 500 mM NaCl, 1 mM EDTA, 0.1 mM DTT and 50% glycerol.
At-A-Glance Documents

Applications

  • Adding poly(A) tails to RNA
  • Labeling the 3'-end of RNA

Components

The B size consists of 5 A sizes (20 U)

Source

E. coli B

Storage

–20°C

Concentration

0.2–2 U/µL

References

  1. Krug, M.S. and Berger, S.L. (1987) Methods Enzymol. 152:316-25.
  2. Winter, G. and Brownlee, G.G. (1978) Nucleic Acids Res. 5:3129-39.

Unit Definition

One unit is defined as the amount of enzyme that incorporates 1 nmol of AMP into tRNA in 10 minutes at 37°C and pH 7.9, using ATP as the substrate.


 
 
Products
Cat. # Product Package Size Price # of Units Select
2180A Poly(A) Polymerase 20 Units $78.00
2180B Poly(A) Polymerase 100 Units $309.00
 

 


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