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Products >  Molecular_Biology >  Modifying_Enzymes >  DNA_Polymerase >  DNA_Polymerase_I_Klenow_Fragment

DNA Polymerase I, Klenow Fragment

DNA Polymerase I, Klenow Fragment (DNA Polymerase I Large Fragment) possesses the 5'–>3' polymerase and 3'–>5' exonuclease activities of intact DNA Polymerase I but lacks the 5'–>3' exonuclease activity of full-length DNA Polymerase I. The lack of 5'–>3' exonuclease activity enables the DNA Polymerase I, Klenow Fragment to be used in random primer labeling and DNA sequencing experiments in addition to second-strand cDNA synthesis. The enzyme is supplied in a buffer of 50 mM potassium phosphate (pH 6.5), 1 mmDTT and 50% glyerol (2140A/B) or ethylene glycol (2140AK/BK).

At-A-Glance Documents Images & Data

Applications

  • Random primer labeling
  • Second-strand cDNA synthesis
  • Dideoxy DNA sequencing

Components

2140A/AK (200 Units)

2140B/BK (5 x 200 Units)

2140AK/BK DNA Polymerase I, Klenow Fragment is a sequencing grade reagent

Source

Recombinant E. coli

Storage

–20°C

 

Unit Definition

One unit is defined as the amount of enzyme required to incorporate 10 nmol of total nucleotides into acid-insoluble product in 30 minutes at 37°C and pH 7.4 using poly d(A-T) as the template-primer.

 

Buffer

Supplied with 10X Reaction Buffer [100 mM Tris-HCl (pH 7.5), 70 mM MgCl2 and 1 mM DTT].

Concentration

2140A/B: 2–5 U/µL

2140AK/BK: 2 U/µL




 
 
Products
Cat. # Product Package Size Price # of Units Select
2140A Klenow Fragment (Large Fragment E. coli DNA Polymerase I) 200 Units $49.00
2140B Klenow Fragment (Large Fragment E. coli DNA Polymerase I) 1,000 Units $198.00
2140AK Klenow Fragment for Sequencing (Large Fragment E. coli DNA Polymerase I) 200 Units $67.00
2140BK Klenow Fragment for Sequencing (Large Fragment E. coli DNA Polymerase I) 1,000 Units $270.00
 

 


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