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Molecular Biology


Products >  Molecular_Biology >  Modifying_Enzymes >  DNA_Polymerase >  DNA_Polymerase_I_E_coli

DNA Polymerase I

DNA Polymerase I catalyzes the incorporation of dNTPs into double-stranded DNA in a 5'–>3' direction that is complementary to the DNA or RNA template strand. It possesses 3'–>5' exonuclease (proofreading) activity as well as 5'–>3' exonuclease activity, making the enzyme useful for DNA end blunting and DNA labeling by nick translation. DNA Polymerase I is supplied in a buffer of 50 mM potassium phosphate (pH 6.5), 1 mM DTT and 50% glycerol.

 

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Applications

  • DNA labeling by nick translation
  • DNA end blunting of 5'- and 3'-overhangs
  • cDNA synthesis from DNA or RNA template

Components

2130A: (500 Units)

2130B: 5 (500 Units) A sizes (2,500 Units total)

Source

Recombinant E. coli

Storage

–20°C

Buffer

Supplied with 10X Reaction Buffer [500 mM Tris-HCl (pH 7.8), 100 mM MgCl2, 1 mM DTT and 0.025% (w/w) BSA].

References

  1. Sambrook, J. et al. (1989) Molecular Cloning: A Laboratory Manual, pp 5.34-5.39, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
  2. Okayama, H. and Berg, P. (1982) Mol. Cell. Biol. 2:161-170.
  3. Rigby, P.W. et al. (1977) J. Mol. Biol. 113:237-251.
  4. Lehman, I.R. et al. (1958) J. Biol. Chem. 233(1):163-170.  
  5. Ricchetti, M. and Buc, H. (1993) EMBO J. 12(2):387-396.
  6. Friedberg, E.C. (2006). Nat. Rev. Mol. Cell Biol. 7(2):143–147. 

Unit Definition

One unit is defined as the amount of enzyme that catalyzes the incorporation of 10 nmol of total nucleotides into acid-insoluble product in 30 minutes at 37°C and pH 7.4, using poly d(A-T) as the template-primer.

Concentration

3–6 U/µL




 
 
Products
Cat. # Product Contents Size Price Units Select
2130A DNA Polymerase I (E.coli) 500 Units $67.00
2130B DNA Polymerase I (E.coli) 2,500 Units $270.00
 

 

 
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