Applications - DNA labeling by nick translation
- Producing blunt ends from 5'- and 3'-overhangs
- Second-strand synthesis of cDNA
ComponentsB size consists of 5 A sizes (500 Units)
Source Recombinant E. coli
Storage –20°C
Buffer Supplied with 10X Reaction Buffer [500 mM Tris-HCl (pH 7.8), 100 mM MgCl2, 1 mM DTT and 0.025% (w/w) BSA].
References - Sambrook, J. et al. (1989) Molecular Cloning: A Laboratory Manual, pp 5.34-5.39, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
- Okayama, H. and Berg, P. (1982) Mol. Cell. Biol. 2:161-70.
- Rigby, P.W. et al. (1977) J. Mol. Biol. 113:237-51.
Unit Definition One unit is defined as the amount of enzyme that catalyzes the incorporation of 10 nmol of total nucleotides into acid-insoluble products in 30 minutes at 37°C and pH 7.4, using poly d(A-T) as the template-primer.
Concentration 3–6 U/µL
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