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Products >  Molecular_Biology >  Gene_Transfer_and_Expression >  Vectors >  pMEI-5_DNA

Retroviral Vector: pMEI-5 Vectors

The pMEI-5 vectors are high expression level retroviral vectors that enable gene transfer into mammalian cells. Following vector transfection into an appropriate packaging cell line, pMEI-5 facilitates transient or stable expression of products containing the psi virus packaging signal as well as the target gene. While the mammalian expression vector pMEI-5 possesses the LTR and psi viral elements, it does not carry the genes necessary for particle formation and replication, namely gag, pol and env. Because the pMEI-5 vector contains an intron with high splicing capacity, this enables a high rate of transcriptional efficiency, with a 2-8 fold higher gene expression level compared with pDON-Al-2 vectors. The pMEI-5 Neo vector offers the neomycin selectable marker for stable product expression.

  At-A-Glance   Documents   Images & Data

Applications

  • Retrovirus-mediated gene transfer into mammalian cells
  • Stable mammalian expression

Storage

–20°C

 

Vector Sizes

  • pMEI-5 Neo DNA: 5,820 bp
  • pMEI-5 DNA: 4,689 bp

Form

10 mM Tris-HCl (pH 8.0), 1 mM EDTA

References

1. Okamoto, S. et al. (2009) Cancer Research 69:9003-9011.



 
 
Products
Cat. # Product Contents Size Price License Units Select
3656 pMEI-5 DNA 20 ug $370.00 License Statements
3655 pMEI-5 Neo DNA 20 ug $370.00 License Statements
 

 

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Cat. # Product Contents Size Price Units Select
3655 pMEI-5 Neo DNA 20 ug $370.00
 

 

 
 

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