This product is a plasmid vector for producing a high expression efficiency retrovirus vector. By transfecting this vector into an appropriate packaging cell line, the vector transiently or stably expresses transcribed products containing the virus-packaging signal (psi), target gene and selective marker.
This vector possesses LTR and psi (viral packaging signal), but not the structural genes necessary for particle formation and replication; sequences gag, pol, and env. Because this vector contains an intron with high splicing capacity, it yields high transcription efficiency. pMEI-5 Neo DNA contains the neomycin resistance gene as a selective marker. Gene expression two- to eight-fold higher than pDON-Al-2 series can be expected.
