pDON-Al-2 is a retroviral vector intended for high-efficiency gene transduction in mammalian cells. This mammalian expression vector contains the psi virus packaging signal and LTR viral element; however, it does not contain the gag, pol and env viral genes required for particle formation and replication. Furthermore, the U3 region of the retroviral vector's 5' LTR is substituted with a more powerful cytomegalovirus-derived promoter, enabling high gene transduction and transcription efficiency as well as the ability to generate high titer recombinant retrovirus. pDON-Al-2 also carries a human actin-derived intron and splice acceptor upstream of the multiple cloning site that increases the efficiency of target gene expression following gene transduction.
pDON-AI-2 Neo DNA mammalian expression vector (Cat.# 3653) encodes a neomycin resistance gene as a drug selection marker.