A retroviral vector for high-efficiency gene transduction. pDON-AI-2 Neo DNA has a neomycin resistance gene as a drug selection marker.
pDON-AI-2 DNA does not contain any MoMLVderived genes (gag, pol, or env coding sequence) except LTR and packaging signal (phi sequence). Its U3 region of 5’ LTR has been substituted with a more powerful promoter derived from cytomegalovirus, giving it a high transcription efficiency and allowing it to be used to generate high titer-recombinant retroviruses and accordingly efficient gene transductions. Moreover, it carries a human actin-derived intron and splice acceptor upstream of the cloning site to increase the efficiency of target gene expression after gene transduction. pDON-AI-2 Neo DNA (Cat.# 3653) has a neomycin resistance gene as a drug selection marker.
