This product is a plasmid vector for producing a retrovirus vector with high transfection, expression efficiency.
By transfecting the vector into an appropriate packaging cell line, the vector expresses transient or stable transcribed product containing viruspackaging signal (psi) and target gene and selective marker.
This vector possesses LTR and psi (viral packaging signal), but not the structural gene necessary for particle formation and replication, which are gag, pol, and env, and this vector contains HCMV IE promoter within U3 region of 5' LTR and neomycin resistant gene as a selective marker.
HCMV IE promoter has high promoter activity, and can produce high-titer retroviruses. Also this vector contains intron with high ability of splicing, and it yields high transcription efficiency.
