A simple gene expression vector for mammalian cells. It can be used to express microRNA precursors and other transcripts as well as ordinary genes. Vectors carry a neomycin-resistance gene or a puromycin resistance gene as a selection marker in mammalian cells.
pBApo-EF1alpha is a simple gene expression vector for mammalian cells. This vector carries a promoter from human polypeptide chain elongation factor (EF-1 alpha promoter) and a polyA signal site from herpes simplex virus thymidine kinase gene. The vectors are useful for the construction of expression plasmids by inserting the ORF of a target gene at the multicloning site. This vector can also be used to express micro RNA precursors and other transcripts in addition to ordinary genes. Furthermore, a cassette of [promoter + ORF + polyA signal] can be easily cut out from this vector and transferred to an adenovirus vector. Adenovirus vectors, which have high infection efficiency and a wide target cell spectrum, are suitable for in vitro and in vivo gene transductions. For constructing recombinant adenoviruses, the Adenovirus Expression Vector Kit (Dual Version) Ver. 2 is recommended.
The pBApo-EF1 alpha series includes vectors carrying a neomycin-resistance gene or a puromycin-resistance gene as a selection marker in mammalian cells.
