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Products >  Molecular_Biology >  Gene_Transfer_and_Expression >  Vectors >  Pyrithiamine_Vectors

Pyrithiamine Vectors

The pPTR vectors are E. coli-Aspergillus shuttle vectors, which contain an ampicillin resistance gene (AmpR) as the selection marker for E. coli and a pyrithiamine-resistance gene (ptrA) as the marker for Aspergillus. Since the multiple cloning site precedes the lacZ region, any DNA insertion into this vector can be easily verified using plates containing IPTG and X-Gal.

At-A-Glance Documents Images & Data

Features

  • pPTR I DNA (4,782 bp) – Chromosomal integration vector for Aspergilis sp.
  • pPTR II DNA (10,032 bp) – Autonomously replicating vector for Aspergilis sp.

Applications

  • Selection of Aspergillus sp. Transformants using Pyrithiamine, which is a Competitive Analog of Thiamine

Notes

As the presence of thiamine decreases the selection efficiency of pyrithiamine media, regents without thiamine should be used with this transformation system.

Manufactured by Hakutsuru Sake Brewing Co., Ltd.

Form

10 mM Tris-HCl (pH 8.0), 1 mM EDTA

Unique Restriction Sites

  • 3621: Hind III, Pst I, Sma I and Kpn I
  • 3622: Hind III, Sma I and Kpn I

Concentration

1 µg/µL




 
 
Products
Cat. # Product Package Size Price # of Units Select
3621 pPTR I DNA 20 ug $282.00
3622 pPTR II DNA 20 ug $282.00
 

 


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