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Products >  Molecular_Biology >  Gene_Transfer_and_Expression >  Synthetic_siRNA_Quantitation_Core_Kit

Synthetic siRNA Quantitation Core Kit

Synthetic siRNA generally has a two deoxythymidine nucleotide (dTT) overhang at its 3’ ends. The Synthetic siRNA Quantitation Core Kit adds polydeoxyadenine (poly dA) residues to the 3’ dTT overhangs and then carries out a reverse transcription reaction with a specially-designed oligo dT primer, allowing preparation of complementary DNA from the synthetic siRNA. High-sensitivity siRNA quantitation of synthetic siRNA can be achieved by using the prepared cDNA as the template in SYBR Green I real time PCR with SYBR Premix Ex Taq II (Tli RNase H plus) or a similar product.

Takara's Synthetic siRNA Quantitation Core Kit is designed for siRNA quantitation when used in combination with real time PCR analysis to assay residual siRNA in total RNA samples extracted from cells, tissues, blood and other specimens. There are two kits available, the Synthetic siRNA Quantitation Core Kit (Cat. #6142) and the Synthetic siRNA Quantitation Kit (with SYBR Premix Ex Taq II Tli) (Cat. #RR861A containing Cat. #6142 and Cat. #RR820A).

Note:

  • This kit is designed for siRNA quantitation of molecules containing 3' d(TT) overhangs.
  • For synthetic siRNA with 3ʼoverhangs other than d(TT), prepare a specific oligo dT primer containing the modified sequence.
  • This product does not work with synthetic siRNAs that carry no 3’ overhangs or with RNA 3’ overhangs.

At-A-Glance Documents Images & Data

Applications

  • siRNA quantitation in total RNA with SYBR Green I Real Time PCR (qPCR)
  • Low copy number siRNA quantitation

Applications

  • siRNA Quantitation

Components

6142 (for 30 reactions)1

Terminal Transferase Buffer390 µL
Terminal Transferase30 µL
dATP150 µL
Recombinant RNase Inhibitor30 µL
Oligo dT Primer2150 µL
RT Buffer270 µL
RT Enzyme Mix30 µL
Universal Primer (10 µM)120 µL
EASY Dilution (for Real Time PCR)1 mL
Control siRNA (20 nM)10 µL
Control Primer (10 µM)20 µL

1 Contains sufficient reagent for 30 poly dA tailing and reverse transcription reactions and sufficient Universal Primer for 120 real time PCRs.
2 Product-specific oligo dT primer

RR861A

Synthetic siRNA Quantitation Core Kit (Cat. #6142)
SYBR Premix Ex Taq II (Tli RNase H plus) (Cat. #RR820A)

Storage

–20°C




 
 
Products
Cat. # Product Package Size Price License # of Units Select
6142 Synthetic siRNA Quantitation Core Kit 30 Rxns $500.00 License Statements
RR861A Synthetic siRNA Quantitation Kit (with SYBR Premix Ex Taq™ II Tli) 1 Set $714.00 License Statements
 

 


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