Synthetic siRNA Quantitation Core Kit is designed to quantify synthetic siRNA when used in combination with real time PCR analysis, and is used to assay the residual amount of the administered siRNA in total RNA samples extracted from cells, tissues, blood, and other specimens. There are two kits available, the Synthetic siRNA Quantitation Core Kit (Cat. #6142) and Synthetic siRNA Quantitation Kit (with SYBR Premix Ex Taq II Tli) (Cat. #RR861A).
Synthetic siRNA generally have two deoxythymidine nucleotides (dTT) as an overhang at the 3’ ends. The Synthetic siRNA Quantitation Core Kit adds polydeoxyadenine (poly dA) to the 3’ overhangs and then carries out a reverse transcription reaction with a specific oligo dT primer, allowing preparation of complementary DNA from synthetic siRNA molecules. High-sensitivity quantitation of the synthetic siRNA can be achieved by using the prepared cDNA as template in a SYBR Green I real-time PCR with SYBR Premix Ex Taq II (Tli RNase H Plus) or a similar product.
Note:
- This kit is designed to quantify synthetic siRNA with deoxythymidines d(TT) as a 3ʼoverhang.
- For synthetic siRNA with 3ʼoverhangs other than d(TT), prepare a specific oligo dT primer with a modified sequence.
- This product does not work with synthetic siRNAs that don't have 3’ overhangs or with RNA 3’ overhangs.
