When coated on surfaces of cell culture dishes, flasks or bags, RetroNectin significantly increases retroviral or lentiviral transduction into mammalian cells. This enhanced gene transfer is thought to occur via the co-localization of retroviral particles and target cells on the RetroNectin molecules. In essence, viral particles bind RetroNectin via interaction with its heparin-binding domain II, while target cells bind RetroNectin primarily through interaction of the cell surface integrin receptor VLA-4 with the fibronectin CS1 site. Additionally, target cells may also bind the RetroNectin recombinant human fibronectin fragment via the interaction of fibronectin tripeptide Arg-Gly-Asp (RGD), located within the molecule's central cell-binding domain, with corresponding cell surface integrin receptor VLA-5.
Improve Viral Transductions with RetroNectin Reagent
Recombinant human fibronectin fragment colocalizes virus and cells to facilitate enhanced gene transfer
- Improves gene transfer efficiency in hard-to-infect cell types and hematopoietic stem cells
- Multivalent molecule simultaneously binds virus particles and cell surface proteins, maximizing cell-virus contact
- Ideal for use with Lenti-X and Retro-X Expression Systems