Takara
login | register | order now | view cart | feedback
Products
Molecular Biology


Products >  Molecular_Biology >  Gene_Transfer_and_Expression >  Aureobasidin_A

Aureobasidin A

Aureobasidin A (AbA) is a cyclic depsipeptide anti-fungal antibiotic with a molecular weight of approximately 1.1 kDa. It is isolated from the filamentous fungus Aureobasidium pullulans R106. The yeast selection marker Aureobasidin A inhibits inositol phosphorylceramide (IPC) synthase, a yeast enzyme that is expressed from the AUR1 gene and is toxic to fungi and yeast at low concentrations (0.1–0.5 µg/mL), including Saccharomyces cerevisiae, Schizosaccharomyces pombe and Candida albicans. Aureobasidin A may be used for stringent drug selection of transformed yeast containing a mutant AUR1-C cassette, which confers resistance to Aurobasidin A, and is used to screen for Aspergillus nidulans transformants with the pAUR316 vector from Takara Bio. A major advantage of Aureobasidin A over conventional nutritional selection markers is that Aureobasidin A offers a much more stringent screen by killing non-transformed cells rather than merely retarding their growth.

At-A-Glance Documents Images & Data Resources

Applications

  • Antibiotic selection of yeast transformants
  • Stringent screening for yeast transformants bearing resistance marker AUR1-C
  • May also be used to select Aspergillus spp. transformants with the pAUR316 vector

Note

For research use only.

Form

Lyophilized. Reconstitute in ethanol or methanol to a stock concentration of 0.5-5.0 mg/mL. Store stock solution at 4°C.

Product is nearly insoluble in water.

References

  1. Takesako, K. et al. (1993) J. Antibiot. 46:1414-1420.
  2. Nagiec, M.M. et al. (1995) J. Biol. Chem. 272:9809-9817.



 
 
Products
Cat. # Product Package Size Price # of Units Select
9000B Aureobasidin A 5 x 10 mg $1,542.00
9000 Aureobasidin A 10 mg $386.00
 

 

Recommended Products
Cat. # Product Package Size Price # of Units Select
3604 pAUR135 DNA 20 ug $282.00
3605 pAUR316 DNA 20 ug $282.00
3600 pAUR101 DNA 20 ug $282.00
 

 



Clontech is a Takara Bio Company © 2014 Privacy Policy | Terms & Conditions