Takara's Adenovirus Expression Vector Kit offers two different methods for recombinant adenovirus production using the same stable cosmid vector. The Full-Length DNA Transfer Method produces recombinant adenoviruses using recombinant cosmids which have been created by direct insertion of the target gene into the cosmid (i.e., no shuttle vector is required). Alternatively, with the COS-TPC method, an adenovirus DNA fragment associated with a terminal protein complex is co-transfected along with a cosmid carrying the target gene into 293 cells. The COS-TPC Method may be performed in situations where, due to the characteristics of the gene, use of the Full-Length DNA Transfer Method may not result in desired recombinant virus production and offers increased efficiency of recombinant adenovirus production (up to two orders of magnitude) as compared to conventional methods.
The Adenovirus Expression Vector Kit and Adenovirus Dual Expression Kit (EF1α) contains components for both Full-Length DNA Transfer and COS-TPC Methods (except DNA-TPC for the COS-TPC Method, which is sold separately).
