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Products >  Molecular_Biology >  DNA_Labeling_Kits >  Random_Primer_DNA_Labeling_Kit

Random Primer DNA Labeling Kit

The Random Primer DNA Labeling Kit, Version 2 is designed for radioactive labeling for hybridization probes. Probe labeling can label DNA with [32P]-alpha-, [35S]-alpha- or [3H]-alpha-dCTP. This random primer DNA labeling kit is based on a modified method by Feinberg and Vogelstein and utilizes random oligonucleotide primers and cloned exonuclease-free E.coli DNA polymerase I, Klenow fragment. Using primers that are 9-mer and longer and exonuclease-free enzyme results in higher labeling efficiency and longer probes. This probe labeling method overcomes many of the disadvantages of conventional nick translation procedures while producing hybridization probes from very small amounts of DNA (10 to 20 ng). The Radioactive Labeling for Hybridization Probes kit can also be used to label DNA fragments that are embedded in low-melting temperature agarose gel slices.
At-A-Glance Documents Images & Data Resources

Applications

  • Probe labeling 
  • Random primer DNA labeling

Components

Random Primer (9-mer)60 µL
10X Buffer75 µL
dNTP Mixture 75 µL
» (0.2 mM each of dATP, dGTP and dTTP)
Exo-free Klenow Fragment (2 U/µL)30 µL
Control DNA Solution (25 ng/mL) 10 µL
» (Lambda DNA digested with Hind III, 25ng/µL)

Reagents required but not included in the kit:

  • Sterilized, distilled H20.
  • TE Buffer (10 mm Tris-HCl, pH 8.0, 1 mM EDTA)
  • Labeled dCTP. This kit is designed to use [alpha-32P]-dCTP (~3000 Ci/mmol); however, [alpha-32S]- and [3H]-dCTP can also be used.

References

1. Feinberg, A. P. and Vogelstein, B. (1983) Anal. Biochem. 132:6-13.

2. Feinberg, A. P. and Vogelstein, B. (1984) Anal. Biochem. 137:266-267.


 
 
Products
Cat. # Product Package Size Price # of Units Select
6045 Random Primer DNA Labeling Kit Ver. 2 30 Rxns $135.00
 

 


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