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Products >  Molecular_Biology >  DNA_Labeling_Kits >  BcaBEST_DNA_Labeling_Kit

BcaBEST DNA Labeling Kit

The BcaBEST DNA Labeling Kit is a novel random primer labeling system that uses longer primers (9-mers) and BcaBEST polymerase, a thermostable DNA polymerase isolated from Bacillus caldotenax. Unlike E. coli Klenow fragment or modified T7 DNA polymerase, Bca polymerase allows stable polymerization of DNA even for templates with high GC content containing highly structured regions. Longer 9-mer primers increase the incorporation efficiency of labeled nucleotides up to 80%.

With this kit, random primer labeling via thermostable Bca polymerase is accomplished in 10 minutes, resulting in high yields of labeled DNA probe. The kit is designed for use with [32P]-alpha-, [35S]-alpha- or [3H]-alpha-dCTP labels, but it can also be used with nonradioactive biotin, digoxigenin, or DNP-labeled dUTPs through dNTP replacement. In general, probes with specific activities of >109 dpm/mg DNA obtained with [32P]-alpha-dCTP (~3,000 Ci/mmol, 111 TBq/mmol).

At-A-Glance Documents Images & Data

Features

  • Random primer labeling in 10 minutes
  • Improved incorporation efficiency especially for templates with complex secondary structure

Applications

  • Random primer labeling of DNA using thermostable Bca polymerase

Components

Random Primer (9-mer)80 µL
10X Buffer 100 µL
dNTP Mixture100 µL
(0.2 mM each of dATP, dGTP, and dTTP)
BcaBEST DNA Polymerase (2 U/µL) 40 µL
(cloned and modified B. caldotenax DNA polymerase)
Control DNA solution (25 ng/µL) 10 µL
(Lambda DNA digested with Hind III, 25 ng/µL)

Note

The kit name has been changed from "Ladderman Labeling Kit" to "BcaBEST Labeling Kit". The kit components have not changed.

 

References

1. Feinberg, A.P. & Vogelstein, B. (1983) Anal. Biochem. 132:6-13.

2. Feinberg, A.P. & Vogelstein, B. (1984) Anal. Biochem. 137:266-267.




 
 
Products
Cat. # Product Package Size Price License # of Units Select
6046 BcaBEST™ Labeling Kit 40 Rxns $157.00 License Statements
 

 


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