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Products >  Molecular_Biology >  Cloning_Mapping_and_cDNA_Synthesis >  pKF3_DNA_Enforcement_Cloning_Vector

pKF3 Plasmid

The Enforcement Cloning System pKF3 plasmid allows for easy detection of recombinant DNA clones without the need to screen bacterial colonies. The pKF3 plasmid DNA contains a chloramphenicol resistance gene (CmR) and the lethal gene streptomycin-sensitive ribosomal protein (rpsL) at downstream regions of the tryptophan promoter/operator. This cloning vector is also designed with a multiple cloning site that possesses an amber mutation and 39 restriction sites on the rpsL gene; rpsL will not be expressed if the target gene is inserted into any of these restriction sites. As a result, only targeted clones (containing both the pKF3 plasmid and the target gene) can be grown on LB medium containing chloramphenicol and streptomycin antibiotics.

At-A-Glance Documents Images & Data

Features

  • Concentration: 500 µg/mL
  • Vector Size: 2,246 bp
  • Form: 10 mM Tris-HCl (pH 8.0), 1 mm EDTA

Applications

  • DNA sequencing using pKF3 primers
  • Enforcement cloning

Purity

  • Contains over 70% double-stranded covalently closed circular pKF3 Plasmid
  • Multiple cloning site integrity confirmed by dideoxy sequencing analysis.
  • Restriction site integrity confirmed by restriction analysis using 39 restriction enzymes.

Storage

–20°C

GenBank

  • Entry Name: ECORPS12B
  • Accession No.: D14641

References

1. Toba-Minowa, M. & Hashimoto-Gotoh, T. (1992) Gene 121:25-33.

2. Hashimoto-Gotoh, T. et al. (1993) Gene 137(2):211-216.


 
 
Products
Cat. # Product Package Size Price # of Units Select
3100 pKF3 DNA 10 ug $57.00
 

 


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