Takara's DNA Ligation Kit is a simple, two component system that enables high efficiency ligation of DNA molecules in vitro. Using an optimized buffer system and T4 DNA Ligase, this DNA ligation kit enables high transformation efficiency ligation in 30 minutes. For general ligation reactions, quick ligation for cloning enables good transformation efficiency in only 3 minutes. Ligation reactions can be used directly in selected bacterial transformations with no need for DNA purification.
Both Takara's DNA Ligation Kit, Version 1 and DNA Ligation Kit, Version 2.1 have a similar format and simple directions, resulting in nearly equivalent high efficiency ligations and transformation efficiencies. The DNA Ligation Kit, Version 2.1 contains a convenient premix of enzyme and buffer that further minimizes pipetting steps and permits final reaction volume minimalization (for standard reactions). By using Solution II of the DNA Ligation Kit, Version 2.1, linker addition ligations may be performed. Transformation efficiency can be further improved by the addition of Solution III (Transformation Enhancer) into circular ligation reactions prior to transformation. The DNA Ligation Kit, Version 1.0 can also be used for DNA concatenation via the addition of Solution B alone.