Takara's DNA Ligation Kits are simple, two-component systems that allow rapid performance of all cloning procedures requiring the covalent joining of DNA molecules in vitro. Using an optimized buffer system and T4 DNA Ligase, these kits allow ligation in 30 minutes with high transformation efficiency. For general ligation reactions, these kits allow good efficiency in only 3 minutes. The reactions can be used directly in some bacterial transformations without DNA purification.
The kits have similar, simple procedures and give nearly equivalent transformation efficiencies. However, Version 2.1 contains a convenient premix of enzyme and buffer that further minimizes pipetting steps and final reaction volume in standard reactions. Solution II of Version 2.1 is designed for linker addition ligations. Transformation efficiency can be improved by the addition of Solution III (Transformation Enhancer) into circular ligation reactions before transformation. Version 1.0 can also be used for DNA concatenation using Solution B alone.
