Choosing a ligation kit requires consideration of several factors. During cloning projects it is helpful to assess whether the ligation involves cloning a long insert, whether rapid ligation would aid overall workflow, and whether the type of ends being ligated are blunt, A-overhang (TA-cloning), or sticky (cohesive). Depending on experimental conditions, transformation efficiency can sometimes be improved by limiting ligation reaction volume and thereby using a smaller volume of ligation mix to transform cells. High-throughput cloning studies may require streamlined ligation reaction protocol approaches to save time and minimize sample handling steps. Takara Bio offers a range of DNA ligation kit options for each of these scenarios. Use this guide to help choose the ligation kit that is best for your purposes.