Takara's DNA Ligation Kit, Mighty Mix, is a one-solution premix ligation reagent that offers high efficiency ligations, particularly for blunt-ended ligation. The basic reaction can be performed quickly for either 5 minutes at 25°C, or 30 minutes at 16°C with equal results for both circular and linear DNA ligations. The 2X Ligation Mix allows small ligation reaction volumes (10 µL) when DNA is limiting, and sufficient reagent for 75–150 ligation reactions. The ligation reaction can be used directly in bacterial transformation.
Note: This kit retains activity through 50 freeze-thaw cycles. The Ligation Mix should be thawed on ice (5–10 minutes) and mixed well by pipette prior to use.
