Takara's DNA ligation Kit LONG is a powerful tool for cloning DNA fragments from 2 kb to over 10 kb in length. The kit contains an optimized ligase/buffer system which enables ligation of long fragments without requiring difficult techniques or special expertise. It is especially well-suited for the construction of BAC libraries.
See accompanying product figures for a comparison of the ligation efficiencies of several commercially available ligation kits with Takara's DNA Ligation Kit LONG in the ligation of DNA fragments of varying sizes (2–18 kb). Takara's LONG Kit gave efficiences of about 50 times higher than the other commercially available DNA ligation kits in ligation of high molecular weight DNA (18 kb) into a cloning vector. However, also note that the DNA Ligation Kit LONG provided superior performance not only with high molecular weight DNA, but also with smaller-size DNA inserts.
