Application- Genomic DNA fragmentation
- dsDNA fragmentation
Components(20 preparations) | Enzyme-1 | 20 µL | | Dilution Buffer-1 | 1,040 µL | | A Solution | 20 µL | | B Solution | 50 µL | | Stop Solution | 400 µL | | 150 mM MgCl2 | 40 µL | | Dilution Buffer-2 | 200 µL | | Enzyme-2 | 20 µL | | 0.5 M EDTA | 50 µL | | dH2O | 10 × 1 mL |
Storage–20°C. Solution A, Solution B, 150 mM MgCl2, 0.5 M EDTA and dH2O may be stored at 4°C.
Materials required but not provided- Thermal Cycler (at least 1 cycler is required; 2 cyclers are preferred)
- Electrophoresis loading buffer
We recommend using a loading buffer that contains a dye (e.g., Orange G) that does not overlap with dsDNA fragmentation bands ranging from 100 bp to 1000 bp in size on the electrophoresis gel. When using BPB or xylene cyanol, please be aware that these dyes overlap with dsDNA fragments in the 100 bp to 1000 bp size range.
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