Home Takara Clontech Logo Takara Clontech
login | register | order now | view cart | feedback
Products
Molecular Biology


Products >  Molecular_Biology >  Cloning_Mapping_and_cDNA_Synthesis >  DNA_Fragmentation_Kit

DNA Fragmentation Kit

The DNA Fragmentation Kit is designed to perform random genomic DNA fragmentation and and other long-chain dsDNA fragmentation via enzymatic treatment followed by blunting of the resulting DNA fragments. The DNA fragmentation kit does not require an additional physical shearing step using an apparatus such as a sonicator. Blunt-end DNA fragments may be ligated with blunt-end vectors. If blunting is not required, the reaction may be stopped after DNA fragmentation is complete.

This kit may also be used to pretreat samples enriched with methylated DNA and to prepare DNA samples for high-speed DNA sequencing.

 

  At-A-Glance   Documents   Images & Data   Resources

Application

  • Genomic DNA fragmentation
  • dsDNA fragmentation

 

 

Components

(20 preparations)

Enzyme-120 µL
Dilution Buffer-11,040 µL
A Solution20 µL
B Solution50 µL
Stop Solution400 µL
150 mM MgCl240 µL
Dilution Buffer-2200 µL
Enzyme-220 µL
0.5 M EDTA50 µL
dH2O10 × 1 mL 

Storage

–20°C. Solution A, Solution B, 150 mM MgCl2, 0.5 M EDTA and dH2O may be stored at 4°C.

Materials required but not provided

  • Thermal Cycler (at least 1 cycler is required; 2 cyclers are preferred)
  • Electrophoresis loading buffer

We recommend using a loading buffer that contains a dye (e.g., Orange G) that does not overlap with dsDNA fragmentation bands ranging from 100 bp to 1000 bp in size on the electrophoresis gel. When using BPB or xylene cyanol, please be aware that these dyes overlap with dsDNA fragments in the 100 bp to 1000 bp size range.




 
 
Products
Cat. # Product Contents Size Price Units Select
6137 DNA Fragmentation Kit 20 Rxns $168.00
 

 

 
 

Clontech is a Takara Bio Company © 2014 Privacy Policy | Terms & Conditions