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Products >  Molecular_Biology >  Cloning_Mapping_and_cDNA_Synthesis >  DNA_Fragmentation_Kit

DNA Fragmentation Kit

This kit is designed to perform random fragmentation of genomic DNAs and other long-chain dsDNAs by enzyme treatment, followed by blunting of the resulting DNA fragments. It does not require physical shearing with an apparatus such as a sonicator. Blunt-end fragments may be ligated with blunt-end vectors. If blunting is not required, the reaction may be stopped after fragmentation is complete.

This kit may also be used to pretreat samples enriched in methylated DNA and to prepare DNA samples for high-speed DNA sequencing.

 
At-A-Glance Documents

Application

  • Perform random fragmentation of genomic DNA and other long-chain dsDNAs.

 

Components

(20 preparations)

Enzyme-120 µL
Dilution Buffer-11,040 µL
A Solution20 µL
B Solution50 µL
Stop Solution400 µL
150 mM MgCl240 µL
Dilution Buffer-2200 µL
Enzyme-220 µL
0.5 M EDTA50 µL
dH2O1 mL × 10

Storage

–20°C.
Note: A Solution, B Solution, 150 mM MgCl2, 0.5 M EDTA, and dH2O may be stored at 4°C.

Materials Required but not Provided

  • Thermal Cycler (at least 1; 2 are preferred)
  • Electrophoresis loading buffer
    We recommend using a loading buffer that contains a dye (e.g., Orange G) that would not overlap with bands for fragments that range in size from 100 bp to 1,000 bp on the electrophoresis gel. When using BPB or Xylene Cyanol, please be aware that they overlap with DNA fragments in this size range.



 
 
Products
Cat. # Product Package Size Price # of Units Select
6137 DNA Fragmentation Kit 20 Rxns $165.00
 

 


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