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Products >  Molecular_Biology >  Cloning_Mapping_and_cDNA_Synthesis >  DNA_Blunting_Kit

DNA Blunting Kit

The DNA Blunting Kit allows the conversion of 3' and 5' overhangs to blunt or flush ends. This conversion is accomplished simultaneously by the 3' to 5' exonuclease and 5' to 3' polymerase activities of T4 DNA Polymerase. The resulting blunt-ended DNA can be ligated efficiently into a vector using the same optimized buffer system employed in Takara's DNA Ligation Kits. The reaction can then be used directly in bacterial transformation or in vitro packaging procedures without need for further DNA purification.

At-A-Glance Documents Images & Data Resources

Application

  • Quick and easy blunting of DNA fragment ends

Components

T4 DNA Polymerase 20 µL
10X Buffer 30 µL
DNA Dilution Buffer 500 µL
Ligation Solution A 2 x 600 µL*
Ligation Solution B 150 µL*

* Ligation solution A and B are also available as DNA Ligation Kit Ver.1.

Storage

–20°C

References

  1. Sambrook J. et al. (1989) in Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Laboratory.



 
 
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