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Products >  IBL >  Miscellaneous >  Secondary_Antibodies >  IgG_H_L

Anti-IgG (H+L) IgG Antibody

These products are affinity-purified IgG antibodies that recognize the heavy and light chains (H+L) of rabbit or mouse immunoglobulin G (IgG) antibodies. They are provided in unlabeled form or as a conjugate with horseradish peroxidase (HRP) enzyme. The antibodies were raised in goat or rabbit using rabbit IgG (H+L) or mouse IgG (H+L), and can be used as a secondary antibody for Western blot (WB) detection, immunohistochemical (IHC) detection, or ELISAs.
At-A-Glance Documents

Features

  • Cat.#: 17502A, 17502B
  • Target Name: Rabbit IgG (H+L)
  • Antigen: Rabbit IgG (H+L)
  • Raised in: Goat
  • Label: HRP
  • Specificity: Cross-reacts with goat, mouse, human, and rat IgG
  • Application: WB, ELISA

  • Cat.#: 17508A
  • Target Name: Mouse IgG (H+L)
  • Antigen: Mouse IgG (H+L)
  • Raised in: Rabbit
  • Label: HRP
  • Specificity: Cross-reacts with goat, rabbit, and human IgG; does not cross-react with rat IgG
  • Application: WB, ELISA

  • Cat.#: 17562A
  • Target Name: Rabbit IgG (H+L)
  • Antigen: Rabbit IgG (H+L)
  • Raised in: Goat
  • Label: None

  • Cat.#: 17568A
  • Target Name: Mouse IgG (H+L)
  • Antigen: Mouse IgG (H+L)
  • Raised in: Rabbit
  • Label: None
  • Specificity: Cross-reacts with goat, rabbit, and human IgG; does not cross-react with rat IgG
  • Application: WB, IHC

  • Cat.#: 17601A, 17601B
  • Target Name: Mouse IgG (H+L)
  • Antigen: Mouse IgG (H+L)
  • Raised in: Goat
  • Label: HRP
  • Specificity: Cross-reacts with rat, goat, rabbit, and human IgG
  • Application: WB, ELISA

  • Cat.#: 17661A
  • Target Name: Mouse IgG (H+L)
  • Antigen: Mouse IgG (H+L)
  • Raised in: Goat
  • Label: None

Applications

Recommended antibody concentration (the optimal concentration should be determined empirically):

  • Western blot: 10–100 ng/mL, except Cat.# 17568, 1–10 µg/mL
  • ELISA: 250–500 ng/mL
  • Immunohistochemical techniques: (Cat.# 17568 only): 1–10 µg/mL

Storage

Lyophilized: stable 5 years at 2–8°C. In solution: stable 2 years at –20°C.

Form

Lyophilized from an antibody solution containing 1% BSA in PBS. To use, reconstitute in 1.0 mL H2O.


 
 
Products
New Cat. # Product Package Size Price # of Units Select
17502A Anti-Rabbit IgG (H+L) Goat IgG Fab' HRP 0.2 mg $550.00
17502B Anti-Rabbit IgG (H+L) Goat IgG Fab' HRP 20 ug $215.00
17508A Anti-Mouse IgG (H+L) Rabbit IgG Fab' HRP 0.2 mg $499.00
17562A Anti-Rabbit IgG (H+L) Goat IgG Affinity Purify 1 mg $535.00
17568A Anti-Mouse IgG (H+L) Rabbit IgG Affinity Purify 1 mg $433.00
17601A Anti-Mouse IgG (H+L) Goat IgG Fab' HRP 20 ug $250.00
17601B Anti-Mouse IgG (H+L) Goat IgG Fab' HRP 0.2 mg $600.00
17661A Anti-Mouse IgG (H+L) Goat IgG Affinity Purify 1 mg $565.00
 

 


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