Thioredoxins are a class of redox proteins found in all organisms. In humans, two isoforms of thioredoxin are encoded by the TXN gene. Thioredoxin is also known as surface-associated sulphydryl protein (SASP), ATL-derived factor (ADF), TRX, TRDX, and TRX1. Thioredoxins are characterized by the presence of two vicinal cysteines in a CXXC motif at the active site. Thioredoxins are maintained in the reduced state by the flavoenzyme thioredoxin reductase, in a NADPH-dependent reaction. Thioredoxins act as electron donors to peroxidases and ribonucleotide reductase. Several transcription factors, such as NF-kappa B and AP1 also require thioredoxin-mediated reduction to exert DNA-binding activity. Knockout of thioredoxin genes in mammals is embryonically lethal, and numerous functions of thioredoxin have been described in defense against oxidative stress, and control of growth and apoptosis.
This kit is a solid-phase sandwich ELISAs using two antibodies that are highly specific to human thioredoxin protein; one is precoated on the ELISA plate and the other is HRP-conjugated. This kit can be used to measure soluble human thioredoxin protein in serum, EDTA-plasma, urine, or cell culture supernatant.
