Tenascin-C (also known as cytotactin, hexabrachion, HXB, J1, neuronectin, glioma-associated-extracellular matrix antigen, and GMEM) is a multifunctional extracellular matrix glycoprotein. It consists of multiple subunits with molecular weights of 210-400 kDa. The subunits contain several repeated motifs, including those with similarity to epidermal growth factor (EGF), fibronectin type III (FNIII), and the globular domain of the beta and gamma chains of fibrinogen. In the embryonic stage, tenascin-C is expressed in migrating cells like the neural crest, and in developing tendons, bone, and cartilage. In adults, studies have suggested that tenascin-C is upregulated during oncogenesis, arthritis, and liver and lung fibrosis.
These kits are solid-phase sandwich ELISAs using two antibodies that are highly specific to nonspliced high molecular weight variants of tenascin-C protein; one is precoated on the ELISA plate, and the other is HRP-conjugated. These kits can be used to measure soluble human (Cat.# 27751) or human/mouse/rat (Cat.# 27767) tenascin-C "large" proteins in serum, plasma, or cell culture supernatants.
