The accumulation of amyloid-beta protein in the cerebral cortex in Alzheimer's disease (AD) is only one of the outcomes of proteolytic cleavage of amyloid precursor protein (APP). Cleavage by beta- and gamma-secretase (amyloidogenic pathway) leads to amyloid-beta production, while cleavage by alpha-secretase (nonamyloidogenic pathway) results in a soluble form of APP, sAPP-alpha.
This kit is a solid-phase sandwich ELISA using two antibodies that are specific to mouse/rat sAPP-alpha protein; one is precoated on the ELISA plate and the other is HRP-conjugated. This assay kit can be used to measure mouse/rat sAPP-alpha in serum, EDTA plasma, cell culture supernatant and brain extract.
