The accumulation of amyloid-beta protein in the cerebral cortex in Alzheimer's disease (AD) is responsible for plaque formation. Amyloid-beta is produced by proteolytic cleavage of amyloid precursor protein (APP), mediated by the enzymes beta- and gamma-secretase. The cleavage reaction produces a number of peptides, ranging from 36 to 43 amino acids in length. Amyloid-beta protein is the main component of plaques found in the brains of patients with Alzheimer's disease. Recent research suggests that soluble, oligomeric forms of amyloid-beta are implicated in the development of Alzheimer's disease. This kit detects human amyloid-beta oligomers that contain two or more epitopes in the N-terminus recognized by the anti-human amyloid-beta (82E1) mouse monoclonal antibody.
This kit is a solid-phase sandwich ELISA using two antibodies that are specific to human amyloid-beta oligomers; one is precoated on the ELISA plate and the other is HRP-conjugated. This assay kit can be used to measure human amyloid-beta oligomers in EDTA-plasma, cerebrospinal fluid, serum, cell culture media or brain extract.