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Glycobiology


Products >  Glycobiology >  Resources >  Glycobiology_Selection_Guide

Tools for Carbohydrate Chemistry, Carbohydrate Analysis, and Glycan Analysis

Glycan analysis often involves structural determination of oligosaccharide components of glycoproteins, glycolipids, glycosphingolipids, and other glycan-containing biological molecules. Sensitive carbohydrate chemistry techniques require specific enzymes such as fucosidase, sialidase, biosidase, and other hydrolytic enzymes with activities specific for certain linkages. Sequential treatment of glycan-containing samples can allow structural determination of the original moiety, a fundamental technique of glycan analysis. Precise, pure enzymes from Takara Bio for carbohydrate chemistry analyses include alpha-1,2 fucosidase, alpha-1,3/1,4 fucosidase, alpha 2,3-sialidase, Lacto-N-biosidase, and glycan hydrolases useful for sphingolipid analysis such as recombinant endoglycoceramidase II. In some situations such as proteomic analysis, it may be desirable to remove oligosaccharide structures prior to analysis of protein fractions by MALDI-TOF mass spectrometry. PNGase (also known as glycopeptidase F) is widely used for this purpose to deglycosylate protein samples. Additional products for glycoprotein research include PALPAK columns designed for separation of carbohydrates by reverse-phase HPLC, the Sialic Acid Florescence Labeling Kit for efficient modification of sialic acid-containing moieties with DMB prior to detection by HPLC, and kits for purification of glycan samples (including hydrazine-treated samples) prior to fluorescent labeling with 2-aminopyridine (pyridylamination reaction). Rely on products from Takara Bio for the sensitivity and specificity needed for accurate carbohydrate analysis.

Products for Carbohydrate Chemistry | Glycan Analysis | Carbohydrate Analysis Selection Guide Glycobiology flow chart
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