The Sialic Acid Fluorescence Labeling Kit is intended for quantitative and highly sensitive sialoglycoconjugate analysis. Neu5Ac, NeuGc, KDN and their O-acetyl derivatives can each be analyzed by this method.
Sialic acids are one of the most important constituents of glycoconjugates in microorganisms and a wide range of higher order animals. Many sialic acids are bound to sugar chains through alpha-ketoside linkages such as alpha-2,3, alpha-2,6 and alpha-2,8. They are heavily involved in cell-to-cell recognition, blood coagulation, fertilization and other biological events.
Conventional methods for sialoglycoconjugate analysis, such as the resorcinol assay and thiobarbituric acid test, involve several complex steps. However, the HPLC-based sialic acid fluorescence labeling kit using 1,2-diamino-4,5-methyleneoxybenzene (DMB) provides a relatively simple and highly sensitive quantitative analysis method. In this method, free sialic acids are analyzed by reverse phase HPLC (PALPAK Type R) after labeling with DMB. Neu5Ac, NeuGc, KDN as well as their O-acetyl derivatives can be individually analyzed through this method, with the detection limit being <57 fmol. Combining DMB labeling with pyridylamino (PA) derivatization results in even higher sensitivity sialoglycoconjugate analysis.