Glycobiology is a field of research that addresses the biological functions of glycans on glycoconjugates such as glycoproteins, glycolipids, proteoglycans and free oligosaccharides, as well as those proteins that specifically interact with glycans (e.g., glycosidases). The pyridylamination reaction was first described in 1978 as a means of analyzing glycan structures1.
The Cellulose Cartridge Glycan Preparation Kit is designed for glycan clean up before pyridylamination of samples. The first step involves packing the fine cellulose microcrystals into the cellulose cartridge. Glycans adhere to the cellulose via hydrophilic interactions during the loading and washing steps. Contaminants such as hydrazinolyzed cell and glyco proteins are removed, along with the enzyme reaction mixture. Finally, the isolated glycans are eluted with ethanol/water.