The Cellulose Cartridge Glycan preparation kit is designed for clean up of glycan samples before pyridylamination.
First, carefully prepared fine cellulose microcrystals are packed in the Cellulose Cartridge. Glycans adsorb to the cellulose by hydrophilic interactions in the loading and washing steps. Contaminants, such as hydrazinolyzed cell and glyco proteins and enzyme reaction mixtures are removed. Finally, isolated glycans are eluted with ethanol/water.