Source

Recombinant E. coli strain

Purity

rEGCase is free from the following exoglycosidase activities: alpha-galactosidase, beta-galactosidase, alpha-N-acetylgalactosaminidase, beta-N-acetylgalactosaminidase, beta-N-acetyl-glucosaminidase, alpha-mannosidase, alpha-fucosidase, and sialidase. This enzyme is also free from glycopeptidase, endo-beta-N-acetyglucosaminidase, proteinase, and sphingomyelinase activities.

Storage

Store at –20°C until use. Thawed solution is stable for 2–3 days at 4°C.

Note

This product contains detergents and is not active without them. Do not use on living cells.

Properties

• Systematic name: Oligoglycosylglucosylceramide glycohydrolase (EC 3.2.1.123)
• Form: Solution in 20 mM sodium acetate (pH 6.), 0.2% BSA, 0.1% Lubrol PX
• Concentration: 100 mU/100 µL
• Optimum pH: 5.0-6.0
• Inhibitors: 1 mM Hg²⁺, Zn²⁺, Cu²⁺
• Not inhibited by: Ba²⁺, Ca²⁺, Mg²⁺ or EDTA

Definition of Activity

One unit of enzyme activity is defined as the amount needed to catalyze the hydrolysis of 1 µmol asialo GM1 per minute.

References

1. Ito, M., and Yamagata, T. (1989) J. Biol. Chem. 264:9510.
2. Park, J.T., and Johnson, M.J. (1949) J. Biol. Chem. 181:149.