Alpha 1, 3/4-L Fucosidase

This enzyme specifically cleaves alpha1,3 and alpha1,4 fucoside bonds, liberating L-fucose from lacto-N-fucopentaose II, lacto-N-fucopentaose III and N-acetyllactosamine type tri-antennary and tetra-antennary sugar chain with alpha1,3 fucoside bonds. It also liberates L-fucose from alpha1-acid glycoprotein.

Applications

Specifically cleaves alpha1,3 and alpha1,4 fucoside bonds

Liberates L-fucose from lacto-N-fucopentaose II, lacto-N-fucopentaose III and N-acetyllactosamine type tri-antennary and tetra-antennary sugar chain with alpha1,3 fucoside bonds

Also liberates L-fucose from alpha1-acid glycoprotein

Source

Streptomyces sp. 142

Storage

Store at –20°C. The stock solution is stable for at least 12 months at 4°C. Avoid repeated freeze-thaw cycles.

Properties

Systematic Name: alpha-L-Fucoside fucohydrolase (E.C. 3.2.1.51)
Molecular weight: 40 kDa
Concentration: 1 mU/mL
K_m (substrate):
- Substrate 1: 10.1 µM (PA-lacto-N-fucopentaose II)
- Substrate 2: 1.08 µM (PA-lacto-N-fucopentaose III)
Optimum pH: pH 6.0 (Substrate 2)
Range of pH stability: pH 4.5–7.0 (37°C, 60 min.)
Stabilizer: 0.1% NP-40, 0.1% Brij-58, and 0.02% BSA

Form

Solution in 50 mM potassium phosphate buffer (pH 6.0) containing 0.1 M NaCI, 0.02% NaN₃, and 0.1% Brij-58

References

Sano, M. et al. (1992) J. Biol. Chem. 267:1522-7.

Definition of Activity

One unit is the amount of enzyme required to hydrolyze 1 µmol of pyridylamino-lacto-N-fucopentaose III within 1 minute at 37°C at pH 6.0.


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