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Products >  Glycobiology >  Glycobiology_Enzymes_and_Reagents >  alpha1-2_L_Fucosidase

N-glycan Degradation: Alpha 1, 2-L Fucosidase

Alpha 1,2-L Fucosidase specifically cleaves the alpha 1,2 fucoside bond, liberating L-fucose from 22-alpha-L-fucosyl lactose (Fuc alpha1-2 Gal beta1-4 Glc) and from porcine gastric mucin oligosaccharides. It also liberates L-fucose from N-acetyllactosamine-type bi-antennary sugar chains with alpha 1,6 fucoside bonds, but the rate of this reaction is extremely slow. Alpha 1,2-L Fucosidase can be used during N-glycan degradation.

  At-A-Glance   Documents   Images & Data

Applications

  • Cleavage of the alpha 1,2 fucoside bond during N-glycan degradation
  • Liberation of L-fucose from 22-alpha-L-fucosyl lactose (Fuc alpha1-2 Gal beta1-4 Glc) and from porcine gastric mucin oligosaccharides

 

Source

Corynebacterium sp.

Storage

Under 4°C and dry conditions. A stock solution of 20 U/mL is stable for at least 3 months at 4°C. The stock solution may be frozen and thawed several times without loss of activity.

Properties

  • Systematic name: alpha-L-fucoside fucohydrolase (E.C. 3.2.1.51)
  • Molecular weight: 43 kDa (gel filtration)
  • K2 (substrate): 4.7 x 10-5 M (p-NP-fucose)
  • Inhibitors: Ag+, Hg2+, Cu2+
  • Optimum pH: pH 8.5 (37°C, 10 min.)
  • Optimum temperature: 34°C (pH 8.5, 10 min.)
  • Range of pH stability: pH 5.5–9.5 (25°C, 60 min.)
  • Thermal stability: Less than or equal to 26°C (pH 8.0, 60 min.)

Form

Lyophilized product (white non-crystalized powder) containing 50 mM potassium phosphate (pH 8.0), 0.5 mM EDTA, and 0.025% sodium azide

References

  1. Sano, M. et al. (1992) J. Biol. Chem. 267:1522-1527.
  2. Sano, M. et al. (1991) Glycoconjugate J. 8:271.

Definition of Activity

One unit is defined as the amount of enzyme required to hydrolyze 1 µmol of p-nitrophenyl alpha-L-fucoside (p-NP-Fucose) in 1 minute at 37°C at pH 8.5.




 
 
Products
Cat. # Product Contents Size Price Units Select
4451 Alpha 1,2-L-Fucosidase 2 Units $339.00
 

 

 
 

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