pha 1, 2-L Fucosidase

Alpha 1, 2-L Fucosidase

This enzyme specifically cleaves the alpha1,2 fucoside bond, liberating L-fucose from 22-alpha-L-fucosyl lactose (Fuc alpha1-2 Gal beta1-4 Glc) and from oligosaccharides from porcine gastric mucin. It also liberates L-fucose from N-acetyllactosamine-type bi-antennary sugar chain with alpha1,6 fucoside bonds, but the rate of this reaction is extremely slow.

Applications

Specifically cleaves the alpha1,2 fucoside bond; liberates L-fucose from 2²-alpha-L-fucosyl lactose (Fuc alpha1-2 Gal beta1-4 Glc) and from oligosaccharides from porcine gastric mucin

This enzyme also slowly liberates L-fucose from N-acetyllactosamine-type bi-antennary sugar chain with alpha1,6 fucoside bonds, but the rate of this reaction is extremely slow

Source

Corynebacterium sp.

Storage

Lower than 4°C under dry condition. A stock solution of 20 U/mL is stable for at least 3 months at 4°C. The stock solution may be frozen and thawed several times without loss of activity.

Properties

Systematic name: alpha-L-Fucoside fucohydrolase (E.C. 3.2.1.51)
Molecular weight: 43kDA (gel filtration)
K₂ (substrate): 4.7 x 10^-5 M (p-NP-fucose)
Inhibitors: Ag⁺, Hg²⁺, Cu²⁺
Optimum pH: pH 8.5 (37°C, 10 min.)
Optimum temperature: 34°C (pH 8.5, 10 min.)
Range of pH stability: pH 5.5–9.5 (25°C, 60 min.)
Thermal stability: Less than or equal to 26°C (pH 8.0, 60 min.)

Form

Lyophilized product (white non-crystalized powder) product containing potassium phosphate, EDTA, and sodium azide

References

Sano, M. et al. (1992) J. Biol. Chem. 267:1522-7.
Sano, M. et al. (1991) Glycoconjugate J. 8:271.

Definition of Activity

One unit is the amount of enzyme required to hydrolyze 1 µmol of p-nitrophenyl alpha-L-fucoside (p-NP-Fucose) within 1 minute at 37°C at pH 8.5.


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