Source

Pseudomonas sp.

Storage

Store at –20°C until use. Store reconstituted solution in aliquots at –20°C. Avoid freeze-thaw.

Properties

• Source: Pseudomonas sp.
• Concentration: 5 mU/µL
• Inhibitors: Hg²⁺, Zn²⁺ and Cu²⁺
• Not inhibited by: MN²⁺, Ca²⁺, Mg²⁺ or EDTA
• Optimum pH: Forward reaction: pH 5.0–6.0 (0.8% TRITON X-100); Reverse reaction: pH 7.0 (0.1% TRITON X-100)

Form

Solution in 50 mM sodium acetate (pH 6.0) containing 0.1% Lubrol PX

References

1. Mitsutake, S. et al. (1998) J. Biochem. (Japan) 123:859-863.
2. Tani, M. et al. (1998) Anal. Biochem. 263:183-188.
3. Mitsutake, S. et al. (1997) Anal. Biochem. 247:52-57.
4. Ito, M. et al. (1995) J. Biol. Chem. 270:24370-4.

Definition of Activity

One unit is defined as the amount of enzyme required to catalyze the hydrolysis of 1 µmol of asialo GM1 per minute.