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Products >  Glycobiology >  Glycobiology_Enzymes_and_Reagents >  SCDase

Sphingolipid Hydrolysis: Sphingolipid Ceramide N-Deacylase (SCDase)

Sphingolipid ceramide N-deacylase (SCDase) is derived from Pseudomonas and hydrolyzes the N-acyl linkage between fatty acids and sphingosine bases in ceramides of various sphingolipids. The enzyme also catalyzes the reverse reaction and possesses transacylation activity. SCDase acts on various acidic and neutral glycosphingolipids and sphingomyelin; however, it exhibits low activity with ceramides.

At-A-Glance Documents Images & Data

Applications

  • Hydrolysis of N-acyl linkages between fatty acids and sphingosine bases
  • Sphingolipid hydrolysis

Source

Pseudomonas sp.

Storage

Store at –20°C until use. Store reconstituted solution in aliquots at –20°C. Avoid freeze-thaw.

Properties

  • Source: Pseudomonas sp.
  • Concentration: 5 mU/µL
  • Inhibitors: Hg2+, Zn2+ and Cu2+
  • Not inhibited by: MN2+, Ca2+, Mg2+ or EDTA
  • Optimum pH: Forward reaction: pH 5.0–6.0 (0.8% TRITON X-100); Reverse reaction: pH 7.0 (0.1% TRITON X-100)

Form

Solution in 50 mM sodium acetate (pH 6.0) containing 0.1% Lubrol PX

References

  1. Mitsutake, S. et al. (1998) J. Biochem. (Japan) 123:859-863.
  2. Tani, M. et al. (1998) Anal. Biochem. 263:183-188.
  3. Mitsutake, S. et al. (1997) Anal. Biochem. 247:52-57.
  4. Ito, M. et al. (1995) J. Biol. Chem. 270:24370-4.

Definition of Activity

One unit is defined as the amount of enzyme required to catalyze the hydrolysis of 1 µmol of asialo GM1 per minute.




 
 
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4462 SCDase (Sphingolipid ceramind N-deacylase) 250 mU $375.00 License Statements
 

 

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